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KMID : 0355620000260040355
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Journal of Korean Association of Oral and Maxillofacial Surgeons
2000 Volume.26 No. 4 p.355 ~ p.362
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Cyclin D1 Gene Amplification in Oral Squamous Cell Carcinoma Using Differential Polymerase Chain Reaction
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1김기순/1Kee Soon Kim
1김경욱/1이재훈/2김창진/1Kyung Wook Kim/1Jae Hoon Lee/2Chang Jin Kim
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Abstract
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-Abstract-
Neoplastic growth is characterized by alterations of oncogenes and antioncogenes. The
interaction between activated oncogenes and functional deletion of antioncogene appears
to be the driving force directing normal cells to uncontrolled growth resulting in tumor.
In addition to those genes mentioned, other genes controlling the entry of cells into the
cell cycle have recently been implicated in cancer development. The overexpression of
the cyclin D1 gene, which has been mapped to 11q13, either by gene rearrangement or
amplification has been noted in various malignant tumors. The product of the cyclin D1
gene forms a complex with cyclin-dependent protein kinases(CDK4) that governs a key
transition in the cell cycle.
The relationships between the overexpression of cyclin D1 assessed by
immunihistochemistry and the amplification of the cyclin D1 gene by differential
polymerase chain reaction(DPCR) using primers for dopamin D2 receptor gene in 13
cases of squamous cell carcinomas of the oral cavity have been studied.
The semiquantitative assay of cyclin D1 amplification has been made by cyclin
D1/dopamin D2 receptor(CD/DR) ratio.
The results were as follows ;
1. In the normal tissue and the tumor, the CD/DR ratios were 0.82 and 1.36
respectively. This implicates 1.65-fold amplification of cyclin D1 gene in tumor compared
to that in normal tissue.
2. The tumor tissue which showed overexpression of cyclin D1 by
immunohistochemistry revealed 2-fold amplification of cyclin D1 compared to the normal
to the normal tissue.
3. The tumor tissue which showed mild expression of cyclin D1 by
immunihistochemistry revealed 1.7-fold amplification of cyclin D compared to the normal
tissue.
4. The cyclin D1 was overexpressed in the tumor tissue at the rate of 38%.
Above results suggest that cyclin D1 has close correlation with the development of
carcinoma in the oral cavity. But further studies were needed to elucidate the
carcinogeneic mechanisms by comparative studies among cyclin D1, pRb and p53.
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